| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103583 |
| Chromosome: | chromosome 7 |
| Location: | 2320404 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327950 | MAW9 | Membrane-associated hydroxyproline-rich glycoprotein 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGTGTCGCTGTTGCCGTACGACCGCAT |
| Internal bar code: | GGCGGGATAACACAAGGAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.903 |
| LEAP-Seq n confirming: | 2060 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCACGTAGGAGCAGACG |
| Suggested primer 2: | GTCCTGGATTACCCTCCCTC |