| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103652 |
| Chromosome: | chromosome_8 |
| Location: | 2385350 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g371000 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGTACTGTCACACATGTGGATTGTGGTGG |
| Internal bar code: | TCAATTGCTCGGCACACAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 98.3347 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCAACCCCTGCTACAAC |
| Suggested primer 2: | CTCTTTCCACGCTTCACACA |