| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103666 |
| Chromosome: | chromosome 7 |
| Location: | 2064594 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325850 | MAW8 | Membrane-associated hydroxyproline-rich glycoprotein 8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGCGACAATGACACGGCCTCCCGCG |
| Internal bar code: | TCAATTTTGACTCGGCTCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 96.8371 |
| LEAP-Seq n confirming: | 2878 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTTTGCCTGACCAAGCC |
| Suggested primer 2: | CGCAATAGCCGAGACCTAAG |