| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103696 |
| Chromosome: | chromosome_6 |
| Location: | 5593516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g285600 | RWP5,MID | RWP-RK transcription factor/minus dominance protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCATTTATGGAAATTCTAGGATCAACGGGA |
| Internal bar code: | GAACAGTCGGAGCCGAATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 98.892 |
| LEAP-Seq n confirming: | 3570 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACGCAGTCTTATGGAAT |
| Suggested primer 2: | ACACATCTGCCCCGACTTAC |