| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103711 |
| Chromosome: | chromosome_6 |
| Location: | 5288124 |
| Confidence (%): | 95 |
| Locus disrupted | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g282800 | ICL1 | Isocitrate lyase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTAACGATTGTTTTGGGAGGACTGTTGC |
| Internal bar code: | TTGACAGTTGCTTTGCGCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.4382 |
| LEAP-Seq n confirming: | 3363 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTCCTGTCACCTCAGA |
| Suggested primer 2: | GCTACTTCGACCTGGTCAGC |