| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103737 |
| Chromosome: | chromosome 10 |
| Location: | 2374283 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435250 | NIC13 | (1 of 1) 6.3.5.1 - NAD(+) synthase (glutamine-hydrolyzing) / Nicotinamide adenine dinucleotide synthetase (glutamine); NAD+ synthase (glutamine-hydrolyzing) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAGTTCCATTGAGGACTGTCGCGGCCG |
| Internal bar code: | TGAGGGTGTCCCCGCCTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 98.1328 |
| LEAP-Seq n confirming: | 473 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGATTTATCGGCGTGT |
| Suggested primer 2: | TCGCATTAGATGCTTGTTGC |