| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103748 |
| Chromosome: | chromosome_16 |
| Location: | 3946254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g690509 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCCTGATTCAGGGCCGTACGGAGATACAG |
| Internal bar code: | TAAGTGGCGGGCCCCTCGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 93.6861 |
| LEAP-Seq n confirming: | 1558 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATACGGGTTTCCCTAAA |
| Suggested primer 2: | TGCTCATCAGCAATTTAGCG |