Insertion junction: LMJ.RY0402.103778_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.103778
Chromosome:chromosome 2
Location:1361553
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g083065 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTGCGTTCTTTGTTCCTCCTCCTCTGC
Internal bar code:AAGTACAACGCAACGGGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:97.35
LEAP-Seq n confirming:1249
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTCAGCATCGCTGTGTG
Suggested primer 2:CACGTGTGGAGCAACAGACT