| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103825 |
| Chromosome: | chromosome_12 |
| Location: | 2573312 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g505350 | ACA2 | P-type ATPase/cation transporter, plasma membrane | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTCGTTCTCGTTCTGAGTCTGTTCTAACT |
| Internal bar code: | ACCTGGGCAGGGCAGGCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.8233 |
| LEAP-Seq n confirming: | 565 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTGGTTGTTTCCTGTT |
| Suggested primer 2: | TACGGACACCACAAGGATGA |