| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103843 |
| Chromosome: | chromosome 7 |
| Location: | 1567608 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324500 | MMP22 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGGCACAGAAGCCACCCCGCGCCCCC |
| Internal bar code: | AGCGTTGAACAGCAGACCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1340 |
| LEAP-Seq percent confirming: | 98.706 |
| LEAP-Seq n confirming: | 1907 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCATGAATATGGTTGCC |
| Suggested primer 2: | TGGTGACAACGTCTGTGGTT |