| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103857 |
| Chromosome: | chromosome 17 |
| Location: | 575672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699900 | AKR10 | Aldo/keto reductase; (1 of 2) PTHR11732:SF255 - ARYL-ALCOHOL DEHYDROGENASE YPL088W-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGACGGGGCGAAGGCAACAAGGGCT |
| Internal bar code: | AATTCGGCGTTACGTGTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 96.6168 |
| LEAP-Seq n confirming: | 1485 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGGAGAACATCGAGGC |
| Suggested primer 2: | TCTTGCTGCACGTCACCTAC |