| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103874 |
| Chromosome: | chromosome 9 |
| Location: | 6587162 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408350 | DeSI-5,DESI5 | DeSI-type SUMO protease; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCCCCGGCTCGATTCGGTGTCCCGGG |
| Internal bar code: | TAGTAGAGCGGCATGAGACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 99.7998 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCTAGGACGCTTTTGT |
| Suggested primer 2: | AATGTGCCTACCTGCACTCC |