| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103884 |
| Chromosome: | chromosome 1 |
| Location: | 7582018 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054750 | (1 of 37) 2.7.11.17 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCGCGGCCTCCAGGACAGTGCCGTCG |
| Internal bar code: | CAATTCGACTGCTGGGGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 99.7771 |
| LEAP-Seq n confirming: | 2238 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCTCTCCGTAAAAGGC |
| Suggested primer 2: | CGCTCGTATCTGAGGTAGGC |