Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.103924 |
Chromosome: | chromosome_12 |
Location: | 2378095 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g507400 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGCACGCAGGCTAGCGACGTGCGCACGGC |
Internal bar code: | GGTTACGGGATAGTCTGTGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 170 |
LEAP-Seq percent confirming: | 76.4084 |
LEAP-Seq n confirming: | 217 |
LEAP-Seq n nonconfirming: | 67 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAGGGCAGAGAAGGGAGAG |
Suggested primer 2: | GGAAAGGGTTGACACCACAT |