| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103925 |
| Chromosome: | chromosome 5 |
| Location: | 765938 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248500 | TRXO,TRXO1,TRX15 | Thioredoxin o, mitochondrial; (1 of 1) PTHR10438//PTHR10438:SF275 - THIOREDOXIN // THIOREDOXIN O1, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGGGCAGGGCAGGAGTCCAGTGCTTG |
| Internal bar code: | CACTGGACTCGCTATCCATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 99.7442 |
| LEAP-Seq n confirming: | 390 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTACACGGTGGTATCTT |
| Suggested primer 2: | GACACATCGCACAACTTGCT |