| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103952 |
| Chromosome: | chromosome 2 |
| Location: | 1202792 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082000 | AGE1 | DNA repair glycosylase; (1 of 1) K03575 - A/G-specific adenine glycosylase (mutY) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCGGAACTGCGCGGTGCAATGCAACGT |
| Internal bar code: | ATCCCGGCAGGGGGAGGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 87.5979 |
| LEAP-Seq n confirming: | 2013 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAATGCTAAAAACGACC |
| Suggested primer 2: | CTGCAGGGAGGTTGATTGTT |