Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.104021 |
Chromosome: | chromosome_10 |
Location: | 1519562 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g428950 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGCGGTGGGGGAGCGGTCGGTGGGGTTGC |
Internal bar code: | GTGAACACACCGGGCACTCGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 739 |
LEAP-Seq percent confirming: | 99.0684 |
LEAP-Seq n confirming: | 3403 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGTCGTGCGTACGTCTTA |
Suggested primer 2: | AACCAATGTCAGCAACCACA |