| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104035 |
| Chromosome: | chromosome 5 |
| Location: | 592750 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232150 | GDH2 | Glutamate dehydrogenase; (1 of 1) 1.4.1.2//1.4.1.3 - Glutamate dehydrogenase / Glutamic dehydrogenase // Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCATGACATTGACGAGTGTTGCATGGG |
| Internal bar code: | TTGTCAACCGTTCGGTTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 99.4735 |
| LEAP-Seq n confirming: | 2834 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAGCTGTAAGGCACCG |
| Suggested primer 2: | TCGACAGGGAATACTCGTCC |