| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104045 |
| Chromosome: | chromosome 16 |
| Location: | 6805697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674739 | (1 of 1) PF12537 - The Golgi pH Regulator (GPHR) Family N-terminal (GPHR_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGGCCGAATCGCGCAGGCCGGCGCCGG |
| Internal bar code: | CCGCTCGACGAGGTCGTTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1119 |
| LEAP-Seq percent confirming: | 99.33 |
| LEAP-Seq n confirming: | 2372 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCAAACTGTTCGACGCT |
| Suggested primer 2: | ATCACTCGCTTGCCTCAGTT |