| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104054 |
| Chromosome: | chromosome 3 |
| Location: | 6360863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193850 | SUCLG1,SCLA1,TEF25,SCL1 | (1 of 1) K01899 - succinyl-CoA synthetase alpha subunit (LSC1); Succinyl-CoA ligase alpha chain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCATGCTAGCTTTTCGAGCACGTGGCG |
| Internal bar code: | CATCGGTGGGCACTCAGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1095 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 1488 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCCATAGCCAACGTG |
| Suggested primer 2: | ACCCAGTACACCGACCTCTG |