| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104055 |
| Chromosome: | chromosome 8 |
| Location: | 328725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358568 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGTCTCGGGGTCGTACGACACAATCA |
| Internal bar code: | CACCCTGGTCGGACGCTCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.4135 |
| LEAP-Seq n confirming: | 678 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCTACTTCAGCTTGGTT |
| Suggested primer 2: | GCAAACTGACGGAGGAGAAG |