| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104061 |
| Chromosome: | chromosome 1 |
| Location: | 2901365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017650 | FAP34,RIB30 | Ribbon binding 30 kDa microtuble inner protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTGCACGGATGTACGTGTCCTGCTCC |
| Internal bar code: | GGCGCAGTTACTGCATGCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 98.7781 |
| LEAP-Seq n confirming: | 4042 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACGTGTTTTGGTCCTCT |
| Suggested primer 2: | TTTCTTATGCAATCCTCCCG |