| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104074 |
| Chromosome: | chromosome 6 |
| Location: | 3756896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278156 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGTCATCGAGGAGCACGTGGAGAGCG |
| Internal bar code: | TGGGAATTTCGAAACTAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.2628 |
| LEAP-Seq n confirming: | 3366 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCTTGTCAACAATGGG |
| Suggested primer 2: | CAGCAATAGTTGTGCTGGGA |