| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104093 |
| Chromosome: | chromosome 13 |
| Location: | 1478282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572450 | (1 of 1) PTHR26402:SF538 - TWO-COMPONENT RESPONSE REGULATOR ARR19-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAATCCATGCTCGATGTCTCCATGTCC |
| Internal bar code: | TGGTTGAGGTACTGCCGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.0621 |
| LEAP-Seq n confirming: | 5070 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAGATGACACGGAGGGG |
| Suggested primer 2: | TCAAGAACATCTGGCAGCAC |