| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104093 |
| Chromosome: | chromosome 13 |
| Location: | 1478283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572450 | (1 of 1) PTHR26402:SF538 - TWO-COMPONENT RESPONSE REGULATOR ARR19-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGCCCTCACGGCTGGAATGGCCCGCG |
| Internal bar code: | TATTAACTGGGACCCGGCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 417 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAGATGACACGGAGGGG |
| Suggested primer 2: | TCAAGAACATCTGGCAGCAC |