Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.104200 |
Chromosome: | chromosome 4 |
Location: | 414760 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g217919 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCGAAGAACCATTTTTATTAAAAGCA |
Internal bar code: | TTAGTAGGGCGCGCGGACCGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 383 |
LEAP-Seq percent confirming: | 93.3036 |
LEAP-Seq n confirming: | 418 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGGGTTTGCACGTGTAGG |
Suggested primer 2: | AGCAGCACATTGTCATCAGC |