| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104227 |
| Chromosome: | chromosome 17 |
| Location: | 4462138 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732300 | GAL1 | (1 of 1) 2.7.1.6 - Galactokinase; Galactose kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAACGCCCCGGGGAGGGAGATGGGTGGA |
| Internal bar code: | CTTCCTTGCTCGCGTGGGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.2941 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGAGGGAGAGGGAGAAT |
| Suggested primer 2: | TATACACCATGCTCGTCCCA |