Insertion junction: LMJ.RY0402.104298_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.104298
Chromosome:chromosome 1
Location:1492207
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g007700 (1 of 1) 3.4.11.1//3.4.11.5 - Leucyl aminopeptidase / Peptidase S // Prolyl aminopeptidase / Proline iminopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGGAACAGCGCGGCGGTGATGGCGCCG
Internal bar code:CGTGAGAGGCGATCAAACTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:99.607
LEAP-Seq n confirming:1774
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACAGGAGAAGTGCG
Suggested primer 2:AAAATGGAATGCGAGGTCTG