Insertion junction: LMJ.RY0402.104337_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.104337
Chromosome:chromosome 1
Location:1975913
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g010700 SYP2 Endosomal Qa-SNARE protein, Syntaxin7/Syx7/Pep12/Syp2-family (Qa.III.b); (1 of 1) PF14523 - Syntaxin-like protein (Syntaxin_2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACATCAAAGGGACAGCTCCATATTCCTC
Internal bar code:AAGAGGGAATCCTGCGCAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:98.3108
LEAP-Seq n confirming:582
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGTCCAGGTTTTGCTT
Suggested primer 2:GTGAGCTTCTCCTTGATGGC