| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104359 |
| Chromosome: | chromosome 14 |
| Location: | 3535520 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630895 | (1 of 1) PTHR13789:SF90 - FAD-BINDING MONOOXYGENASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTGCGGCTGTGGTTCATCACGGTGTC |
| Internal bar code: | GCGCGGGTCAGACTGGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 98.3977 |
| LEAP-Seq n confirming: | 2088 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACTGCTGGTTGACGAC |
| Suggested primer 2: | GGCTTGGCTAGGACTCACTG |