| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104368 |
| Chromosome: | chromosome 1 |
| Location: | 7973686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g068377 | (1 of 1) K14771 - U3 small nucleolar RNA-associated protein 19 (NOC4, UTP19) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTACCAAGAGCACAGCGCCTCAGAAACG |
| Internal bar code: | GTCATTTAGGGAGATGCTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.7273 |
| LEAP-Seq n confirming: | 1097 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACAGGGGTTTGAGCATC |
| Suggested primer 2: | CACACAGTGCACAACACTGC |