| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104399 |
| Chromosome: | chromosome 9 |
| Location: | 5194943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399252 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCAGACGGCGTGCCGATAGGGGCAGG |
| Internal bar code: | TGACCTTACAGCTGCATGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 98.8558 |
| LEAP-Seq n confirming: | 3715 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACCTTCTGGGTGTCGAT |
| Suggested primer 2: | GTTCGAAGACAGCGATGTGA |