| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104400 |
| Chromosome: | chromosome 6 |
| Location: | 1705236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261400 | DTH1 | Delayed in Triacylglycerol Hydrolysis 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGACTCTCGCCTGAGCCTTCCTCGACGC |
| Internal bar code: | TCGGGCTTTTTCACTTGTAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGAGCGACATACATGG |
| Suggested primer 2: | GACACGCTGTCTTTGATGGA |