| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104400 |
| Chromosome: | chromosome 6 |
| Location: | 1719407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261500 | CPLD58 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR11260:SF136 - THIOREDOXIN FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGCCGACTCCAAAACTCCACAAGCCCA |
| Internal bar code: | CCAGGGGCCACTTGGTACGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 98.5104 |
| LEAP-Seq n confirming: | 992 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGGTGAGCTACGTGTG |
| Suggested primer 2: | AACATGACACAGCGCTCATC |