| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104413 |
| Chromosome: | chromosome 8 |
| Location: | 4362273 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381702 | ALDH5,ALDH5G1 | (1 of 1) 1.2.1.16 - Succinate-semialdehyde dehydrogenase (NAD(P)(+)); Aldehyde dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTGCTCGTCAGCTGTTGATGGCGACTC |
| Internal bar code: | TATGCCTCCAGCTGGCCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 98.6156 |
| LEAP-Seq n confirming: | 2137 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTCGTGTGTTTGCAGTT |
| Suggested primer 2: | TTACGCACATGAGTCGAAGC |