| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104422 |
| Chromosome: | chromosome 17 |
| Location: | 3274729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723050 | (1 of 8) PTHR23023//PTHR23023:SF130 - DIMETHYLANILINE MONOOXYGENASE // INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCTTGACGCTGCCGCCGCAAAGTTGCT |
| Internal bar code: | GGTGGGCAAGTCGTAATAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 99.1346 |
| LEAP-Seq n confirming: | 3322 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTAACCGCTCCATTGCTC |
| Suggested primer 2: | GGCTACACCAGCATGCATAA |