| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104441 |
| Chromosome: | chromosome 2 |
| Location: | 4136913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098000 | EBM2 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCCATTTTTTCCCACCCAGCCGCCCG |
| Internal bar code: | TTTTACGGTTGTTCGGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 99.0587 |
| LEAP-Seq n confirming: | 2210 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAGAGAGACGAAGGGGG |
| Suggested primer 2: | GCTACGAGGAGTGGAACTCG |