| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104463 |
| Chromosome: | chromosome 9 |
| Location: | 1702406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396900 | (1 of 1) K03426 - NAD+ diphosphatase (E3.6.1.22, NUDT12, nudC); Nudix phosphohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCCGTGCATGCCTCTCCTCCCCTCCCT |
| Internal bar code: | GTTTCATAGAAAGCGGGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCAACCAACCAACCAAC |
| Suggested primer 2: | TAGCCCCACCAAGTAACCAG |