| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104470 |
| Chromosome: | chromosome 7 |
| Location: | 4027624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340050 | (1 of 1) K14537 - nuclear GTP-binding protein (NUG2, GNL2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATGCAAGGATAGTTGACTGGTGCATAC |
| Internal bar code: | GTCGCCGCGTCCTTGGCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 98.5604 |
| LEAP-Seq n confirming: | 4998 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCAGGAAGGACAGACG |
| Suggested primer 2: | GCATGTTTTAACACCGCCTT |