| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.104493 |
| Chromosome: | chromosome 17 |
| Location: | 2526000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716400 | (1 of 1) K14816 - pre-60S factor REI1 (REI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTCGGCCGAGGGCCAGGAGACCCTAGC |
| Internal bar code: | TACGCACTCCTCTTCAGCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 97.6783 |
| LEAP-Seq n confirming: | 10602 |
| LEAP-Seq n nonconfirming: | 252 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCCAACCTGGAGTACAT |
| Suggested primer 2: | ACAGCCCAAACAATACCTGC |