Insertion junction: LMJ.RY0402.104506_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.104506
Chromosome:chromosome 12
Location:8156246
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g551050 (1 of 1) PTHR31802//PTHR31802:SF3 - FAMILY NOT NAMED // 32 KDA HEAT SHOCK PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACAGAGACGAGTCAATCTTTCGGAACTT
Internal bar code:TCTAAGTAGTCTTTCCCTTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGACACGTGTGTAGAGG
Suggested primer 2:AATGGCTGTGGGTTTACTGC