| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104556 |
| Chromosome: | chromosome 1 |
| Location: | 6215547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044450 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCAGCGAAGCAGATTGCTGAGCAGCAA |
| Internal bar code: | TTCGAATAACCATGCTTCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 112 |
| LEAP-Seq percent confirming: | 88.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGAGCTGACGGAGAAC |
| Suggested primer 2: | TATTGCGCAGAGACAAAACG |