| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.104576 |
| Chromosome: | chromosome_1 |
| Location: | 4328232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029350 | C1d-87,FAP297 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTTCTGCCGGGCTGCAGGCTCCAAGGGC |
| Internal bar code: | TGCCCGCGCGGTCCCTTCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 99.5086 |
| LEAP-Seq n confirming: | 405 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCTATCCAACACGCAC |
| Suggested primer 2: | CATTTTGCTTGTGGTGTTGG |