| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104579 |
| Chromosome: | chromosome 3 |
| Location: | 4364626 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175200 | TOC75 | Translocon at the outer envelope membrane of chloroplasts, 75 kDa subunit; (1 of 2) PTHR12815//PTHR12815:SF7 - SORTING AND ASSEMBLY MACHINERY SAM50 PROTEIN // OUTER ENVELOPE PROTEIN 80, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGCCCACCCACTGACCGTGCGTCATT |
| Internal bar code: | AGGTGGGAATAGCACCGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 58.7184 |
| LEAP-Seq n confirming: | 953 |
| LEAP-Seq n nonconfirming: | 670 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTTGTACACCACCCGCT |
| Suggested primer 2: | AAGCAACTTGTGGTTCTGGG |