| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104619 |
| Chromosome: | chromosome 2 |
| Location: | 7079117 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147100 | FAP265 | Flagellar Associated Protein 265; (1 of 7) PF05517 - p25-alpha (p25-alpha) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGTCGGGCGAAGGTGCTTGCACAACCG |
| Internal bar code: | CTCTTGTCTAGGTATACGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.7639 |
| LEAP-Seq n confirming: | 845 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACACTAAGCACTGCCAA |
| Suggested primer 2: | CTCCCTTCATTCTAAGCCCC |