| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104641 |
| Chromosome: | chromosome 16 |
| Location: | 1973711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656500 | ALA3 | (1 of 1) K01530 - phospholipid-translocating ATPase (E3.6.3.1); ATPase, phospholipid transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGACGCGCCGCCGCAAGCTCCGACAGT |
| Internal bar code: | TGTCGCCTCGGTGGGTCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1267 |
| LEAP-Seq percent confirming: | 99.6431 |
| LEAP-Seq n confirming: | 3071 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCACTTCTCTGTACCAA |
| Suggested primer 2: | GTGTACACAAACCGGAGGCT |