| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104648 |
| Chromosome: | chromosome 1 |
| Location: | 4715839 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032500 | DNJ8,TPR2 | Tetratricopeptide-repeat protein 2; (1 of 1) K09523 - DnaJ homolog subfamily C member 3 (DNAJC3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTGCTGCTGCTGCATGGCTCGGTTGG |
| Internal bar code: | TTATTGCTGCGGTTACGTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 99.1859 |
| LEAP-Seq n confirming: | 2315 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACAGCTGCTGCATAACA |
| Suggested primer 2: | TGTGACACCCTTGCATGTTT |