| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.104664 |
| Chromosome: | chromosome 2 |
| Location: | 5576895 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108900 | CAM6 | Calmodulin-like protein; (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAAACGCAACAACGCACTGCAACGCTC |
| Internal bar code: | ACCGATCGGTCAGGCATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 92.5774 |
| LEAP-Seq n confirming: | 2033 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGTGTTGTGCATGTAGG |
| Suggested primer 2: | GTCCTCACAATCCCCATACG |