| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104681 |
| Chromosome: | chromosome 11 |
| Location: | 3178754 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479383 | (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGGTCGGTGCTGCGTTGTGGAAGAGC |
| Internal bar code: | AAAAATTATCTAATCCTCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.0991 |
| LEAP-Seq n confirming: | 1100 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGAGGTGCGTTGCTATG |
| Suggested primer 2: | CGGGATCCAAGACCAGAGTA |