| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104741 |
| Chromosome: | chromosome 9 |
| Location: | 3240674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387726 | AST1 | Aspartate aminotransferase; (1 of 1) K14454 - aspartate aminotransferase, cytoplasmic (GOT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTCCGGCGGGGCCTGCGGAGGAGAGA |
| Internal bar code: | ACGTCGTAAGAGTCTGACAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 98.3736 |
| LEAP-Seq n confirming: | 2359 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACGAAGACGCTAAGACC |
| Suggested primer 2: | ACATGGAAAGGAAGTCACGG |